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A Practical Guide for the Laboratory

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Microscopic Haematology 3rd Ed

A fully-updated edition of the ultimate haematology textbook for diagnostic use 

The third edition provides over 400 full colour haematological slides of exceptional quality.

Arranged in a logical order, it commences with the red cell series describing normoblastic erythropoiesis and then goes on to describe abnormal erythropoiesis and all the red cell disorders associated with anaemia. Each type of anaemia is described with a minimal amount of text and accompanied by coloured haematological slides depicting the red cell changes associated with the particular disorder. The images have been magnified x 1000 and are of a very high quality. The platelet section follows on and adheres to the same format.

Microscopic Haematology - A practical guide for the laboratory by Gillian Rozenberg. There is a section on haematology relating to paediatrics which describes red cell, white cell and platelet disorders occurring in cord blood, the neonate and childhood. The last section is on Blood Parasites and describes the four species of human malaria. A description of the characteristic features of each species as it occurs in the red cell is accompanied by images clearly depicting the various stages of maturation of each species of malaria.

Microscopic Haematology Book Cover

Review 1

Reviewed by:

Darryl Graham, MAIMS
National Laboratory Operations Manager, IDEXX Laboratories
3 Overend Street, EAST BRISBANE, QLD 4169
darryl-graham@idexx.com

Publication review appeared in:

Australian Journal of Medical Science
May 2011, Vol. 32 No. 2

Microscopic Haematology:

A Practical Guide for the Laboratory (3rd Edition)
Author: Gillian Rozenberg
Publisher: Churchill Livingstone
Format: Soft cover, 256 pages, over 400 colour illustrations
ISBN: 978-0-7295-4072-8
RRP: $135.00

Review:

This is the third edition of this most professionally and expertly presented atlas of haematology. The first edition was published in 1996 followed by the second in 2003. Once again, a standout feature of the book is the exceptionally high-quality illustrations. The supporting text is thorough yet concise and always relevant.

The third edition includes 92 additional images detailing cell morphology and ultra-structure, resulting in over 400 photomicrographs of slides in total. There is also online access for students to free learning resources and activities to supplement the learning material in the book (Evolve). For instructors, there is online access to case studies relating to the illustrations contained in the book that would be very helpful as a teaching tool.

Gillian Rozenberg FAIMS is an acknowledged authority on blood cell morphology and has once again utilized her knowledge and expertise to produce an invaluable volume for reference in the medical laboratory and for students in the field of haematology.

This book contains four sections. The first section includes erythropoiesis, anaemias, haemoglobin disorders, membrane disorders, and miscellaneous. The second section, dealing with leucocytes and platelets, covers maturation, abnormal cells, neoplasms, and includes special stains where warranted. The third section on paediatric haematology covers cord blood, red cell disorders, bone marrow failure, benign disorders of leucocytes, myeloproliferative neoplasms, non-haemopoietic malignancies, storage disorders, and platelet abnormalities. The fourth section deals with blood parasites, including the four generally recognized species of human malaria and also Plasmodium knowlesi, now recognized as a fifth species infecting humans, as well as non-malarial blood parasites.

The text in all sections includes accurate and succinct descriptions, and the illustrations are always relevant and have been reproduced to represent faithfully what would be seen when viewed microscopically. In the main, the detail of the slides is excellent, and the cell structure/inclusions are very clear; however, some of the illustrations for the fourth section dealing with malarial parasites are a little too small for enough detail to be seen.

The descriptions of neoplasms in this edition are classified according to the generally accepted fourth edition of the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. Cytogenetic and cytochemical/immunophenotypic details are included where applicable.

Having spent much of my career in the Haematology Department of a large metropolitan children's hospital, I know too well that paediatric haematology, both normal and abnormal, is significantly different from that seen in adults. The inclusion of a chapter dedicated to the detailed description of paediatric haematology is most welcome.

The book does not purport to be a haematology textbook, and nor is it. The minimalist texts assist the student of haematology in identifying and recognizing the morphological features; however, there is little or no explanation of the underlying cellular and physiological mechanisms which give rise to those morphological abnormalities. For example, the shortened red cell life span in lead poisoning is mentioned but not elucidated.

Perhaps references to suitable texts could be incorporated. Labelling of the features in the photomicrographs would also be a welcome improvement. In addition, a glossary of terms would add value; for example, burr cells appear in figure A3-17 with no previous mention or description.

Recommendation: I would recommend this practical guide as an absolute must for all laboratories and teaching institutions. It provides an exceptional resource for remote laboratories and will prove invaluable for training students who will appreciate the e-learning feature and the book’s very competitive price. The compact size makes the book easy to use next to the microscope, especially when compared to other atlases.

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Review 2

Reviewed by:

Valerie L. Ng, PhD
(University of California San Francisco)
for Doody's Electronic Journal

Microscopic Haematology:

A Practical Guide for the Laboratory, 2nd Edition
By Gillian Rozenberg

Description

This is the second edition of a comprehensive atlas of the microscopic appearance of a variety of disorders detected in the peripheral blood and bone marrow.

Purpose

The purpose is to provide a comprehensive guide to the microscopic appearance of a variety of hematological disorders and other disorders that cause abnormalities in the peripheral blood or bone marrow. Improvements from the first edition include the addition of pediatric hematology as well as use of the World Health Organization (WHO) classification of primary hematological disorders. The author has clearly succeeded in her worthy goals.

Audience

This book would be very useful for practicing clinical laboratory scientists (CLSs), pathologists, and clinical hematologists. It would be of interest to medical and CLS students, pathology/laboratory medicine residents, and anyone interested in clinical microscopy.

Features

This half-inch thick, paperback book contains a wealth of hematology information. The photomicrographs are absolutely first-rate, with excellent color and definition. Everything that could possibly be detected in peripheral blood—from primary malignant hematological disorders to congenital hematological disorders to infectious agents—is represented in this book.

An extremely helpful feature is the use of the WHO classification for primary hematological disorders, accompanied by a brief description of the associated immunophenotypic and karyotypic abnormalities. The pediatric hematology section was a nice addition and is also very well done.

There are few things that captivate a hematologist/hematopathologist as much as a beautiful and comprehensive atlas of microscopic images of the vast spectrum of abnormalities detected by clinical microscopy. This book will astound and please many a hematologist/hematopathologist. I was personally awestruck with the sheer depth and breadth of this book as well as the beauty of the photomicrographs.

Assessment

This is one of the best annotated microscopic atlases for disorders detected in the peripheral blood or bone marrow that I've encountered to date. Its small size and ready portability make it that much more appealing. Get it!

Weighted Numerical Score: 97 - ★★★★★

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Review 3

Reviewed by:

Ms. Robyn Wells, MAIMS
(QHPS-RBHc Haematology Dept - Herston, Queensland)

Microscopic Haematology:

A Practical Guide for the Laboratory, 2nd Edition
By Gillian Rozenberg

Overview

Microscopic Haematology: A Practical Guide for the Laboratory was first published in 1996. The second edition has been well received, with its high-quality illustrations and clear, concise text. Its publication is timely, as it describes the neoplastic diseases of the lymphoid and haemopoietic tissues using the recently introduced and now almost universally accepted World Health Organization (WHO) classification.

This edition has been expanded to include a section on paediatric haematology covering cord blood, red cell disorders, bone marrow failure, benign disorders of leucocytes, myeloproliferative disorders, non-haemopoietic malignancies, storage disorders, and platelet abnormalities.

Content & Classification

As stated by the author, a full description of the WHO classification of the neoplastic diseases of the lymphoid and haemopoietic tissues is beyond this book’s scope. However, the author does an excellent job of reducing this system of classification to its essential elements and presenting it in a very logical and ordered way, making it readily comprehensible to the reader.

The book also indicates when the WHO classification corresponds to the French-American-British (FAB) classification. All relevant cytogenetics and immunophenotypes commonly used to form a diagnosis are included.

Illustrations & Presentation

The photomicrographs are of a very high standard, representing what you would expect to see under the microscope for a given disease or disorder. The quality of printing on good paper ensures that subtle nuclear and cytoplasmic details are clearly visible.

The entire book features clear, concise text written in easy-to-read plain English. It presents relevant information with very few wasted words. The illustrations have been carefully selected to complement the text, and the index is cross-referenced to help readers quickly find information.

Assessment

It is difficult to find fault with this book. Perhaps future editions could include references, as it is not only a practical guide for the laboratory but also a valuable teaching text in tertiary institutions.

Final Verdict: This book is a worthwhile purchase for anyone with an interest in morphology or who needs a quick reference for haematological conditions.

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Cases in Microscopic Haematology

Cases in Microscopic Haematology is a collection of 80 case studies specifically designed to engage the learner in the laboratory process of data and blood film analysis, differential diagnosis and reporting.

Cases in Microscopic Haematology Book Cover

Each case provides the family history, analyser data and blood film/slide. Students use a template to complete the differential diagnosis and reporting. The process and template simulates the laboratory environment. Cases are organised according to difficulty and the worked cases are available in the back of the text.

A suite of 20 Virtual Slides will accompany the casebook. Cases in Microscopic Haematology is an excellent companion to Microscopic Haematology: a practical guide for the laboratory 3e and will appeal to both the academic and professional market as either a pack or stand-alone resource.

This text will provide the opportunity for the student and laboratory technician to work through the case studies using a template similar to that used in a functioning laboratory.

Features


Example Case Study

Case 20: A 25 year-old Asian female who is 4 weeks pregnant. The analyser data and the blood film indicate that this patient has a microcytic hypochromic anaemia.

Q: What is your differential diagnosis? What tests would you request by the clinician to arrive at an actual outcome or definitive diagnosis?

November 2011 ISBN: 978 0 7295 4092 6 Spiral Bound, 218pp Churchill Livingstone

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Guide to Paediatric Haematology Morphology (2024)


Buy Now

This illustrated guide to identifying or confirming blood disorders in paediatric patients presents examples of the abnormal morphology involved. Clinicians in both haematology and paediatrics will find this an invaluable resource.

Guide to Paediatric Haematology Morphology Book Cover

Table of Contents

Introduction

Examination of the Blood Film. Preparation of the film. Examination of the film. Artefactual changes seen on the blood film. White cell artefact. Poor staining. Crush artefact. Platelet artefact. Red cell classification. Significance of the red cell distribution width (RDW).

Section 1: Red Cells Erythrocytes in the neonate and childhood: Are they macrocytic, normocytic, or microcytic (why the change in size?). Foetomaternal haemorrhage. The art of blood film morphology. Red cell reference ranges. Reticulocyte reference ranges. Electron microscopic image of normal red cells. Cord blood. Anaemia in the neonate. ABO incompatibility. Rh haemolytic disease of the newborn. Twin to twin haemorrhage prior to birth. Erythroblastosis fetalis. Haemoglobin disorders. The a thalassaemias. Silent carrier a-thalassaemia trait. a- thalassaemia trait. Haemoglobin H disease. Haemoglobin H disease cresyl blue. Hydrops fetalis. Haemoglobin constant spring (HbCS). The β thalassaemias. Silent carrier β thalassaemia trait. β-thalassaemia trait. β-thalassaemia intermedia. β-thalassaemia major. Abnormal haemoglobins. Haemoglobin C. HBC trait. HBCC disease. In vitro test for detection of HBC. Haemoglobin E. HBE trait. HBEE disease. Hb E/thalassaemia. Hb E/β thalassaemia. Hb S/β thalassaemia. HB haemoglobin S. HBS trait. HBSS disease. In vitro sickling test for detection of HBS. Red cell membrane disorders. Herederitary spherocytosis. Hereditary elliptocytosis. South-east Asian ovalocytosis. Heredeitary stomatocytosis (Hydrocytosis). Hereditary xerocytosis. Heredeitary pyropoikilocytosis (HPP). Abetalipoproteinaemia. Vitamin E deficiency. Liver disease. Burns (third degree). Diamond blackfan anaemia (DBA). Haemolytic anaemias. Haemolytic anaemia dure to lead poisoning. Oxidant-drug-induced haemolytic anaemia. Pyruvate kinase (PK) deficiency. Autoimmune haemolytic anaemia (AIHA). Microangiopathic haemolytic anaemia. Valvular heart disease. Haemolytic uraemic syndrome (HUS). Thrombotic thrombocytopenic purpura (TTP). Marfan’s syndrome. Disseminated intravascular coagulation (DIC). Malignancy. HELLP syndrome. Paroxysmal cold haemoglobinuria (PCH). Congenital sideroblastic anaemia. Transient erythroblastopenia of childhood (TEC). Recovert from TEC. Miscellaneous red cell images. Splenectomy - Howell Jolly bodies. Splenectomy – Acanthocytes. Lipaemic plasma.

Section 2: White Cells White cell reference ranges in infancy and childhood. Myeloid maturation. Myeloblast. Promyelocyte. Myelocyte. Metamyelocyte. Band form. Neutrophil. Eosinophil. Basophil. Abnormal Myeloid Cells. Pelger-Huët anomaly. Hypersegmented neutrophil. Hypergranulated neutrophils. Toxic vacuolation. Döhle bodies. Leukaemoid reaction. Kawasaki disease. Alder-Reilly anomaly. Mucopolysaccharidosis Type VI (MPS VI). Chédiak-Higashi anomaly. Basophilia/Mastocytosis. Cutaneous mastocytosis (CM). Mast cell leukaemia (MCL). Neonatal neutrophilia. Sepsis in the neonate. Bone marrow failure. Aplastic anaemia. Dyskeratosis congenita (DC). Pancytopenias. Fanconi anaemia (FA). Shwachman-Diamond syndrome (SDS). Neutropenia. Cyclic neutropenia. Kostmann syndrome. Eosinophilia. Eosinophilia in the neonate. Eosinophilia in early childhood. Leucoerythoblastosis. Osteopetrosis. Myeloproliferative neoplasms in the neonate and childhood. Transient abnormal myelopoiesis (TAM). Monocytes and macrophages. Monocytic maturation. Monoblast. Promonocyte. Monocyte. Gaucher disease. Niemann-Pick disease. Reactive haemophagocytic syndrome. Langerhans cell histiocytosis (LCH). Storage disorders in the neonate and childhood. a-Mannosidosis. Mucopolysaccharidoses. Hurler syndrome (Gasser lymphocytes). Cystinosis. Wolman disease. Monosomy 7 myeloproliferative disease (MPD). Cytogenetics. Juvenile myelomonocytic leukaemia (JMML). Cytogenetics. Myelodysplastic syndromes (MDS). Lymphocytes. Lymphocyte maturation. Lymphoblast. Prolymphocyte. Lymphocyte (small). Lymphocyte (large). Reactive lymphocytosis. Reactive lymphocytes (Infectious mononucleosis) (IM). Cytomegalovirus (CMV) infection. Varicella infection. Viral hepatitis. Bordetella pertussis. Acute infectious lymphocytosis. Sialic acid storage disease. Non-haemopoietic malignancies in the neonate and childhood. Neuroblastoma. Rhabdomyosarcoma. Ewing sarcoma.

Section 3: Platelets Platelet reference ranges in infancy and childhood. Megakaryocytic maturation. Megakaryoblast. Promegakaryocytes. Megakaryocyte. Platelet abnormalities. Reactive thrombocytosis. Large and giant platelets. Platelet aggregates. Platelet satellitism. Thrombocytopenia. Thrombocytopenia due to increased destruction (ITP). Thrombocytopenia due to impaired or ineffective thrombopoiesis. Amegakaryocytic thrombocytopenia (AMEGA). Bernard-Soulier syndrome (BSS). Gray platelet syndrome (GPS). May-Hegglin anomaly (MHA). Thrombocytopenia with absent radii (TAR). Wiskott-Aldrich syndrome (WAS). Thrombocytosis. Lymphoproliferative neoplasms. B lymphoblastic leukaemia/lymphoma. T-lymphoblastic leukaemia. Immunophenotype. T lymphoblastic leukaemia/lymphoma. Immunophenotype.

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